Created by principles, and IP, of Cell and Tissue Systems, the primary focus of T3LLC is on low cost to market development of cryopreservation methods to provide human cell-based in vitro systems for high throughput screening purposes. T3 utilizes proprietary methods for preservation of human cell-derived monolayers and 3D cultures in multiwell plates. T3 is positioned to take advantage of the current trend away from animal testing for cosmetics, household chemicals and drugs by providing cryopreserved human cell-based tissue equivalents in place of current practice just in time manufacturing. The longer-term focus of T3LLC is research and development of ice-free cryopreservation methods for clinical applications. Such applications include major internal organs, larynx, trachea, abdominal wall, knee tissues, skeletal muscles, facial tissues and limb extremities, as well as critical natural and engineered tissues such as skin, heart valves and blood vessels. T3LLC also offers consulting and contract research services to commercial and academic organizations with an emphasis on living biological material transport and cryopreservation.