
ICE-Free Vitrification and Nano Warming Technology for Banking of Cardiovascular StructuresAward last edited on: 5/22/2023
Sponsored Program
SBIRAwarding Agency
NIH : NHLBITotal Award Amount
$2,045,742Award Phase
2Solicitation Topic Code
837Principal Investigator
Kevin G M BrockbankCompany Information
Tissue Testing Technologies LLC (AKA: T3LLC~T3 LLC)
2231 Technical Parkway Suite A
North Charleston, SC 29406
North Charleston, SC 29406
(843) 514-6164 |
N/A |
www.t3-tissuetestingtechnologies.com |
Location: Single
Congr. District: 06
County: Charleston
Congr. District: 06
County: Charleston
Phase I
Contract Number: 1R44HL142455-01A1Start Date: 5/1/2019 Completed: 10/31/2020
Phase I year
2019Phase I Amount
$365,069Public Health Relevance Statement:
NARRATIVE There are huge markets for research, diagnostic and clinical applications of naturally occurring and engineered cells, tissues and organs. Strategic assessment of the field has identified the need for better preservation methods because freezing methods of cryopreservation have been shown to damage tissues and organs due to ice formation. This proposal focuses on nanowarming technology development for cryopreservation by vitrification of large volume blood vessels samples. Nanowarming is required for viable, functional preservation of blood vessels. There are significant clinical needs for vascular grafts for dialysis as well as patients requiring peripheral and coronary bypass grafts. Advances in the preservation of tissues are also needed for trauma care, particularly to incorporate regenerative medicine products into strategic national stockpiles. This proposal combines the use of novel cryoprotectant formulations with magnetic nanoparticles and radiofrequency- induced warming to warm optimally vitrified, banked, living, biological materials. These technologies could eventually impact hundreds of thousands of patients in North America annually if applied to tissues, tissue engineered cellular constructs and one day organs.
NIH Spending Category:
Bioengineering; Biotechnology; Cardiovascular; Nanotechnology; Regenerative Medicine; Transplantation
Project Terms:
Achievement; Animal Model; Animals; Aorta; Area; Arteries; base; Bathing; Biocompatible Materials; Biological; Biological Assay; Biomechanics; Blood Preservation; blood vessel transplantation; Blood Vessels; Blood Volume; Cardiovascular system; Carotid Arteries; Cell Survival; Cells; cellular engineering; Chemistry; Clinical; clinical application; clinically relevant; Controlled Environment; Convection; Coronary Artery Bypass; cryogenics; Cryopreservation; Cryopreserved Tissue; crystallinity; Crystallization; cytotoxicity; Detection; Diagnostic; Dialysis procedure; Diffuse; Disaccharides; Dry Ice; Electromagnetic Fields; Endothelium; Engineering; Evaluation; Excision; experience; experimental study; Exposure to; Extracellular Matrix; Family suidae; femoral artery; Formulation; Fracture; Freezing; Fresh Tissue; Future; Generations; Glass; Heating; Histopathology; Human; human tissue; Hyperplasia; Ice; In Vitro; in vivo; Inflammation; innovation; Laser Scanning Microscopy; Lead; Liquid substance; Logistics; Lung; Magnetic nanoparticles; Magnetic Resonance Imaging; Market Research; Measurement; Mechanics; Medial; Medicine; Metals; method development; Methods; Microscopic; microwave electromagnetic radiation; Modeling; nano; nanoparticle; nanowarming; Nitrogen; nonhuman primate; North America; novel; novel strategies; Organ; Outcome; packaging material; Patients; Peripheral; Permeability; Phase; phase change; physical state; Physiological; practical application; pre-clinical; preclinical evaluation; preservation; pressure; prevent; Property; Protocols documentation; Pulmonary artery structure; radio frequency; Raman Spectrum Analysis; Regenerative Medicine; Reproductive Medicine; resazurin; Research; Residual state; response; Rewarming; Sample Size; Sampling; Scanning Electron Microscopy; second harmonic; Shipping; Small Business Innovation Research Grant; Solid; Specimen; Structure; Surface; System; Techniques; Technology; technology development; Temperature; Testing; Thick; Thinness; Time; Tissue Engineering; Tissue Preservation; Tissue Viability; Tissues; Transition Temperature; Translations; transplant model; Transplantation; trauma care; vapor; Vascular Graft; Work
Phase II
Contract Number: 4R44HL142455-02Start Date: 11/1/2020 Completed: 3/31/2023
Phase II year
2021(last award dollars: 2022)
Phase II Amount
$1,680,673Public Health Relevance Statement:
NARRATIVE There are huge markets for research, diagnostic and clinical applications of naturally occurring and engineered cells, tissues and organs. Strategic assessment of the field has identified the need for better preservation methods because freezing methods of cryopreservation have been shown to damage tissues and organs due to ice formation. This proposal focuses on nanowarming technology development for cryopreservation by vitrification of large volume blood vessels samples. Nanowarming is required for viable, functional preservation of blood vessels. There are significant clinical needs for vascular grafts for dialysis as well as patients requiring peripheral and coronary bypass grafts. Advances in the preservation of tissues are also needed for trauma care, particularly to incorporate regenerative medicine products into strategic national stockpiles. This proposal combines the use of novel cryoprotectant formulations with magnetic nanoparticles and radiofrequency- induced warming to warm optimally vitrified, banked, living, biological materials. These technologies could eventually impact hundreds of thousands of patients in North America annually if applied to tissues, tissue engineered cellular constructs and one day organs.
Project Terms: