A program of research and development is described to develop a fully integrated biofluidic system-on-chip hosting multiplexed DNA, protein and cellular analysis. A novel methodology of on-chip flow programming, enabled by Light-controlled Electrokinetic Assembly of Particles near Surfaces (LEAPS) is introduced along with requisite tools for biofluidic chip design. Fabrication technologies and architectures are identified which support the progressive integration of on-chip flow programming and microfluidic dispensing of sample and reagents from individually programmable reservoirs with a novel optically programmable bead array technology which accommodates polymeric microbeads as well as cells in a highly parallel assay format. Specific applications of this integrated biofluidic processor to cellular functional assays include cytokine profiling as a broad-band indicator of infection.
