Date: Jun 01, 2015 Source: Company Data (
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Engineering Arts working in close collaboration with the Arizona State University Biodesign Institute, Virginia G. Piper Center for Personalized Diagnostics (VGPCPD) has developed the HD-NAPPA protein microarray platform. HD-NAPPA allows proteins to be expressed in-situ from DNA printed into (1"x3") silicon nanowell (SiNW) devices instead of the more conventional and problematic process of generating and printing purified proteins on coated glass. Two key technologies / instruments have been developed and installed in VGPCPD lab.
1) Advanced piezoelectric printing instrument which enable us to target and functionalize batches of SiNW devices with thousands of unique protein coding DNA reagents.
2) Assay apparatus to fill and seal the SiNW devices with bulk reagents for diffusion free protein synthesis.
The platform is used in applications for biomarker identification, drug target discovery, and protein-protein interaction studies.
Our current working density allows us to screen serum samples against greater than 20,000 biomarkers with a single 1x3" SiNW device. Several studies using HD-NAPPA are currently in process at VGPCPD. The following peer review publications describe the technology and completed studies in more detail:
High Density Diffusion-Free Nanowell Arrays (featured on cover)
Bharath R. Takulapalli, Ji Qiu, D. Mitchell Magee, Peter Kahn, Al Brunner, Kristi Barker, Steven Means, Shane Miersch, Xiaofang Bian, Alex Mendoza, Fernanda Festa, Karan Syal, Jin G. Park, Joshua LaBaer, and Peter Wiktor
Journal of Proteome Research 2012 11 (8), 4382-4391
DOI: 10.1021/pr300467q (2013)
Antiviral Antibody Profiling by High-density Protein Arrays
Bian, X., Wiktor, P., Kahn, P., Brunner, A., Khela, A., Karthikeyan, K., Barker, K., Yu, X., Magee, M., Wasserfall, C. H., Gibson, D., Rooney, M. E., Qiu, J. and LaBaer, J.,
Proteomics, 15: 2136--2145.
doi: 10.1002/pmic.201400612 (2015)
Microreactor Array Device
Peter Wiktor, Al Brunner, Peter Kahn, Ji Qiu, Mitch Magee, Xiaofang Bian, Kailash Karthikeyan and Joshua LaBaer
Scientific Reports 5, Article number: 8736 doi:10.1038/srep08736 (2015)
We gratefully acknowledge the NIH, NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES for supporting this effort. Grant Number: 5R42RR031446-02
Patent Pending with ASU AzTE.
For further information on HD-NAPPA and collaboration opportunities contact: Peter Kahn
