GenHunter Corporation is developing more streamlined mRNA Differential Display technology to the biomedical research community. Living organisms have thousands, to tens of thousands, of unique genes encoded in their genome and only a small fraction, perhaps 15%, is expressed in any individual cell. The course of normal cellular development as well as pathological changes that arise in diseases like cancer are believed to be driven by gene expression changes. mRNA Differential Display technology works by systematic amplification of the 3' terminal portions of mRNAs and resolution of those fragments on a DNA sequencing gel. The differential display method can visualize all the expressed genes in a eukaryotic cell in a systematic and sequence-dependent manner using multiple primer combinations. The technology is finding wide-ranging and rapid applications in fields such as developmental biology, cancer research, neuroscience, pathology, endocrinology and plant physiology.
