SBIR-STTR Award

A cost effective bacterial seed treatment for seed rot
Award last edited on: 5/21/2002

Sponsored Program
SBIR
Awarding Agency
USDA
Total Award Amount
$228,000
Award Phase
2
Solicitation Topic Code
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Principal Investigator
E Wayne Vinje

Company Information

Bozeman Bio-Tech

Po Box 3146 1612 Gold Avenue
Bozeman, MT 59715
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Location: Single
Congr. District: 00
County: Gallatin

Phase I

Contract Number: ----------
Start Date: 00/00/00    Completed: 00/00/00
Phase I year
1994
Phase I Amount
$50,000
Seed rot caused by the fungus pythium ultimum is a major cause of crop loss in northern climates where cool planting seasons are common. Seeds are generally treated with the fungicides metalaxyl and captan (banned in germany) to prevent seed rot. A biotype of the bacteria pseudomonas aureofaciens -- ab 254 -- has performed as well as metalaxyl in preventing seed rot for sweet corn and beets, and, at times, for peas and beans. It appears th ep. Aureofaciens ab 254, perhaps in combination with other bacterial strains, can protect a range of other commercial crops. P. Aureofaciens is a saprophytic species, not known to be pathogenic, and ab 254 is inexpensive to produce. Questions on methods for bio-treatment of some crops and on large scale, commercial seed treatment processes using these bacteria remain. In this project Montana State University will extend ab 254 and related bio-types to equivalent chemical control performance for crops other than sweet corn. Bozeman bio-tech will define, simulate and test cost effective processes for large scale, commercial seed treatment with ab 254 and related bacteria application mechanisms, including consideration of bio-priming and bacterial coating with and without combined polymer/other coating constituents.

Phase II

Contract Number: ----------
Start Date: 00/00/00    Completed: 00/00/00
Phase II year
1995
Phase II Amount
$178,000
The saprophytic Pseudomonas aureofaciens AB 254 equals chemical protection of sweet corn against the most common seed rots Psthium optimum. Phase I field tests provided similar results for cucurbitaceous crops, confirming AB 254 performance sufficient for commercialization. AB 254 was not effective against Rhizoctonia solani, a secondary seed rot for fungi. Formulation for shelf life, not proposed but undertaken, identified freeze drying and air dried processes providing acceptable AB 254 survival, 29% - 44%. An AB 254, carboxymethyLcellulose coating exhibited - 5 months viability, sufficient for one season. Coating seeds with freeze dried AB 254 will cost $0.15/lb versus S0.44/lb for safe film fungicide coating or $0.05/lb for hazardous chemical coatings. In Phase II we will identify an 18 month shelf life formulation, concentrating on freeze drying. We will isolate an AB 254 companion bacteria effective against R. solani. The commercial processes to produce formulate and apply AB 254. and its companion bacteria, will be simulated and the results field tested. We will define the AB 254 protection mechanisms: e.g., antibiosys, parasitism/lysis and competition. to support shelf life formulation and AB 254 extension to other crops. Finally. we will estimate EPA registration costs and refine market and cost analysis.Applications: