SBIR-STTR Award

This Small Business Innovation Research (SBIR) Phase I project proposes to apply Multi Photon Detection technique (MPD) to quantify proteins bound to a microarray of antibodies. The first arrays will target cytokines, the important secreted proteins that regulate the immune system. The goals of this project are to create an immunoassay based protein-chip for the concurrent measurment of all the cytokines down to the level of a few zeptomole/ sample, to implement improved P-chip designs using peptide nucleic acid linkers, and to study the levels of cytokines in cell cultures and human samples. By analyzing patterns of protein expression and their post-translational modifications, correlations of functions and/or of disease states with specific protein expression patterns are expected to be established. The commercial applications of this project are in the area of medical diagnostics.

This Small Business Innovation Research Phase II project proposes to develop a novel supersensitive multiphoton detection system for protein chips (P-chip/MPD) for applications in drug discovery and in early detection of prostate cancer and breast cancer. The commercial impact of the proposed work will be in the area of diagnostic proteomics. The diagnostics industry is large, currently estimated at around 10 billion dollars per year. The most profitable and dynamically growing fields are those that permit early detection of cancer and therapy monitoring, or provide toxicity assays for new drugs. It is expected that the P-Chips/MPD developed in this project will eventually capture a significant share of the diagnostic proteomics market. PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH: Note: When clicking on a Digital Object Identifier (DOI) number, you will be taken to an external site maintained by the publisher. Some full text articles may not yet be available without a charge during the embargo (administrative interval). Some links on this page may take you to non-federal websites. Their policies may differ from this site. K-H Kim, A Dobi, S Shaheduzzaman, CL Gao, K Masuda1, H Li, A Drukier, Y Gu, V Srikantan1, JS Rhim and S Srivastava "Characterization of the androgen receptor in a benign prostate tissue-derived human prostate epithelial cell line: RC-165N/human telomerase reverse transcriptase" Prostate Cancer and Prostatic Diseases , v.10 , 2007 , p.30 Ksenya Shchors, Elena Shchors, Fanya Rostker, Elizabeth R. Lawlor, Lamorna Brown-Swigart, and Gerard I. Evan "The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1b" GENES & DEVELOPMENT , v.20 , 2006 , p.2527 Andrzej K. Drukier, Nathasha Ossetrova, Elena Schors, Galya Krasik, Ivan Grigoriev, Christina Koenig, Miko Sulkowski, Jerzy Holcman, Larry R. Brown, John E. Tomaszewski, Mitchell D. Schnall, Richard Sainsbury, Anne E. Lokshin, and Jasminka Godovac-Zimmerm "High-Sensitivity Blood-Based Detection of Breast Cancer by Multi Photon Detection Diagnostic Proteomics" Journal of Proteome Research , v.5 , 2006 , p.1906 Andrzej K. Drukier,Natascha Ossetrova, Elena Schors, Larry R. Brown, John Tomaszewski,Richard Sainsbury, and Jasminka Godovac-Zimmermann "Ultra-Sensitive Immunoassays Using Multi-Photon-Detection in Diagnostic Proteomics of Blood" Journal of Proteome Research , v.4 , 2005 , p.2375 Andrzej K. Drukier,Ivan Grigoriev, Larry R. Brown, John E. Tomaszewski, Richard Sainsbury, and Jasminka Godovac-Zimmermann "Looking for Thom?s Biomarkers with Proteomics" Journal of Proteome Research , v.5 , 2006 , p.2046 Anna E. Lokshin, Zoya Yurkovetsky, Brian Nolen, Adele M. Marrangoni, Lydmila Velikokhatnaya, Andrzej Drukier, Steven Skates, Elieser Gorelik "Multimarker assay for early diagnosis of ovarian cancer" American Association for Cancer Research , v.47 , 2006 , p.653 Richard Sainsbury, Andre Drukier, Natascha Ossetrova, John Tomaszewski, Jasminka Godovac-Zimmermann, Elena Schors "Ultra-sensitive immunoassays using multi-photon-detectionin diagnostic proteomics of blood" American Association for Cancer Research , v.47 , 2006 , p.2873 Oliver Kleiner, David A. Price, Natasha Ossetrova, Sergei Osetrov, Peter Volkovitsky, Andrzej K. Drukier, Jasminka Godovac-Zimmermann "Ultra-high sensitivity multi-photon detection imaging in proteomics analyses" Proteomics , v.5 , 2005 , p.2322