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SBIR-STTR Award
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SBIR-STTR Award
10
Method for Increasing the Hybridization Efficiency of DNA and RNA Probes
Award last edited on: 11/4/02
Sponsored Program
SBIR
Awarding Agency
NSF
Total Award Amount
$219,931
Award Phase
2
Solicitation Topic Code
-----
Principal Investigator
Karin D Rodland
Company Information
Pacific Biotechnology Research Associate
8000 SE 35th Avenue
Portland, OR 97202
(503) 777-1674
N/A
N/A
Location:
Single
Congr. District:
03
County:
Multnomah
Phase I
Contract Number:
8460645
Start Date:
00/00/00
Completed:
00/00/00
Phase I year
1984
Phase I Amount
$33,384
A method for increasing the sensitivity of nucleic acid hybridization reactions involving non-radioactive DNA probes is examined. Pacific Biotechnology Research Associates has a proprietary method which increases the hybridization efficiency of radioactively-labeled genetic probes by over 1000%. This method will be applied to commercially-available systems which produce non-radioactive DNA probes to determine whether the same degree of amplification can be obtained in a non-radioisotopic system. Several modifications of the basic technique will be tested in order to determine the optimal condition for increasing the signal obtained with non-radioactive DNA probes.The potential commercial application as described by the awardee: The widespread application of recombinant DNA technology to commercial use in genetic engineering or diagnosis is currently limited by the safety and expense considerations of radioactively-labeled genetic probes, and the relative insensitivity of non-radioactive probes. Development of a generic method to improve probe hybridization efficiencies will substantially facilitate the commercial exploitation of genetic probes.
Phase II
Contract Number:
8521281
Start Date:
00/00/00
Completed:
00/00/00
Phase II year
1987
Phase II Amount
$186,547
___(NOTE: Note: no official Abstract exists of this Phase II projects. Abstract is modified by idi from relevant Phase I data. The specific Phase II work statement and objectives may differ)___ A method for increasing the sensitivity of nucleic acid hybridization reactions involving non-radioactive DNA probes is examined. Pacific Biotechnology Research Associates has a proprietary method which increases the hybridization efficiency of radioactively-labeled genetic probes by over 1000%. This method will be applied to commercially-available systems which produce non-radioactive DNA probes to determine whether the same degree of amplification can be obtained in a non-radioisotopic system. Several modifications of the basic technique will be tested in order to determine the optimal condition for increasing the signal obtained with non-radioactive DNA probes.The potential commercial application as described by the awardee: The widespread application of recombinant DNA technology to commercial use in genetic engineering or diagnosis is currently limited by the safety and expense considerations of radioactively-labeled genetic probes, and the relative insensitivity of non-radioactive probes. Development of a generic method to improve probe hybridization efficiencies will substantially facilitate the commercial exploitation of genetic probes.
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