Multi-omics profiling of individual exosomes for their origin-tracing, biomarker discovery, and biological function characterization
Award last edited on: 5/19/2023

Sponsored Program
Awarding Agency
Total Award Amount
Award Phase
Solicitation Topic Code
Principal Investigator
Yuchao Chen

Company Information

Wellsim Biomedical Technologies Inc

200 Lincoln Centre Drive
Foster City, CA 94404
   (814) 777-8586
Location: Single
Congr. District: 14
County: San Mateo

Phase I

Contract Number: 1R43GM145015-01
Start Date: 3/1/2022    Completed: 2/28/2023
Phase I year
Phase I Amount
Exosomes, the small extracellular vesicles (30-200 nm), are highly heterogeneous in biofluids. However,traditional bulk-level analysis approaches fail to represent their individual variations. A technique for multi-omicsprofiling of exosomes at single-particle resolution is of great interest to the exosome research field, but has yetto be developed. WellSIM proposes to develop and validate a method for multi-omics profiling of individualexosomes based on our exosome isolation system (EXODUS), a droplet-based microfluidic device (EXOSeq),and next-generation sequencing (NGS). Our proposed technique will be the first integrative proteomic andtranscriptomic profiling of exosomes at the single-particle level, offering high-resolution multi-dimensionalbiological insights for exosome study and application.

Public Health Relevance Statement:
PROJECT NARRATIVE The conventional exosome characterization approaches usually analyze entire exosome populations, which cannot reveal the exosome heterogeneities or quantify exosome subpopulations in biofluids. Our proposed technique could address the unmet need for multi-omics single-exosome studies, offering substantial benefits to the EV research community. Its unprecedented resolution and sensitivity for exosome interrogation will facilitate exosome-based biomarker discovery and detection. This technique will also enable characterization of exosome subpopulations and their tissues of origin, as well as improve our understanding of exosome biogenesis and biological functions.

Project Terms:

Phase II

Contract Number: ----------
Start Date: 00/00/00    Completed: 00/00/00
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