Exosomes, the small extracellular vesicles (30-200 nm), are highly heterogeneous in biofluids. However,traditional bulk-level analysis approaches fail to represent their individual variations. A technique for multi-omicsprofiling of exosomes at single-particle resolution is of great interest to the exosome research field, but has yetto be developed. WellSIM proposes to develop and validate a method for multi-omics profiling of individualexosomes based on our exosome isolation system (EXODUS), a droplet-based microfluidic device (EXOSeq),and next-generation sequencing (NGS). Our proposed technique will be the first integrative proteomic andtranscriptomic profiling of exosomes at the single-particle level, offering high-resolution multi-dimensionalbiological insights for exosome study and application.
Public Health Relevance Statement: PROJECT NARRATIVE
The conventional exosome characterization approaches usually analyze entire exosome populations, which
cannot reveal the exosome heterogeneities or quantify exosome subpopulations in biofluids. Our proposed
technique could address the unmet need for multi-omics single-exosome studies, offering substantial benefits to
the EV research community. Its unprecedented resolution and sensitivity for exosome interrogation will facilitate
exosome-based biomarker discovery and detection. This technique will also enable characterization of exosome
subpopulations and their tissues of origin, as well as improve our understanding of exosome biogenesis and
biological functions.
Project Terms:
