
DNA-based assay for calorimetric determination and quantitation of protein concentrations in pure or mixed solutionsAward last edited on: 3/20/2023
Sponsored Program
SBIRAwarding Agency
NIH : NIGMSTotal Award Amount
$258,625Award Phase
1Solicitation Topic Code
859Principal Investigator
Matthew W EskewCompany Information
Phase I
Contract Number: 1R43GM146542-01A1Start Date: 9/6/2022 Completed: 6/5/2023
Phase I year
2022Phase I Amount
$258,625Public Health Relevance Statement:
Project narrative:
Production of many biopharmaceuticals or biologics, low abundance and engineered molecules is accomplished using prokaryotic or eukaryotic expression systems; allowing for the manufacture of a large variety of biological compounds on a small scale for screening applications, and large scale on the metric-ton scale for therapeutics. However, while expression of the targeted biological compounds is cheap and fast, a major drawback is the time required and expense involved in purification and isolation of targeted compounds. We have developed an assay that will allow quantitative analysis of proteins prior to purification; in this project we will screen biologically expressed SARS-CoV-2 proteins from a commercial collaborator for validation studies.
Project Terms:
Antibodies, Biological Assay, Assay, Bioassay, Biologic Assays, Biological Products, Biologic Products, Biological Agent, biologics, biopharmaceutical, biotherapeutic agent, Buffers, Differential Scanning Calorimetry, Calorimetric Differential Thermal Analysis, Clinical Trials, Culture Media, growth media, DNA, Deoxyribonucleic Acid, Pharmaceutical Preparations, Drugs, Medication, Pharmaceutic Preparations, drug/agent, Engineering, Environment, Equipment, Feedback, Fluorescence, Glass, In Vitro, Laboratories, Libraries, Methods, Painful, Pain, Peptides, Production, Proteins, diagnostic kit, test kit, Diagnostic Reagent Kits, Reagent, Research Resources, Resources, Mass Photometry/Spectrum Analysis, Mass Spectrometry, Mass Spectroscopy, Mass Spectrum, Mass Spectrum Analyses, Mass Spectrum Analysis, Survey Instrument, Surveys, Testing, Thermodynamic, Thermodynamics, Time, Viral Proteins, Viral Gene Products, Viral Gene Proteins, virus protein, Work, base, Vial device, Vial, Phase, Biological, biologic, Individual, Measurement, Collaborations, Therapeutic, instrument, Complex, Scanning, Techniques, System, Nucleocapsid Proteins, experience, melting, novel, validation studies, Sampling, Property, response, Complex Mixtures, preventing, prevent, Address, protein structures, proteins structure, protein structure, Molecular Target, Protein Analysis, Scheme, Characteristics, Process, Modification, Instruction, cost, design, designing, Outcome, Consumption, Peptide antibodies, novel therapeutics, new drug treatments, new drugs, new therapeutics, new therapy, next generation therapeutics, novel drug treatments, novel drugs, novel therapy, data reduction, screening, 2019-nCoV, 2019 novel corona virus, 2019 novel coronavirus, COVID-19 virus, COVID19 virus, CoV-2, CoV2, SARS corona virus 2, SARS-CO-V2, SARS-COVID-2, SARS-CoV-2, SARS-CoV2, SARS-associated corona virus 2, SARS-associated coronavirus 2, SARS-coronavirus-2, SARS-related corona virus 2, SARS-related coronavirus 2, SARSCoV2, Severe Acute Respiratory Coronavirus 2, Severe Acute Respiratory Distress Syndrome CoV 2, Severe Acute Respiratory Distress Syndrome Corona Virus 2, Severe Acute Respiratory Distress Syndrome Coronavirus 2, Severe Acute Respiratory Syndrome CoV 2, Severe Acute Respiratory Syndrome-associated coronavirus 2, Severe Acute Respiratory Syndrome-related coronavirus 2, Severe acute respiratory syndrome associated corona virus 2, Severe acute respiratory syndrome corona virus 2, Severe acute respiratory syndrome coronavirus 2, Severe acute respiratory syndrome related corona virus 2, Wuhan coronavirus, coronavirus disease 2019 virus, coronavirus disease-19 virus, hCoV19, nCoV2, ACE2, angiotensin converting enzyme 2, angiotensin converting enzyme II
Phase II
Contract Number: ----------Start Date: 00/00/00 Completed: 00/00/00