Unlike C. elegans and mice, methods for cold storage of zebrafish lines are lacking in the marketplace. We propose the use of environmental and gene c factors to enable cold storage of viable embryos at frozen or near freezing conditions. In Aim 1 we will explore the use of transient protein expression to produce proteins associated with anhydrobio c status. In Aim 2 we explore the induc on of genes involved in diapause in killfish as a mechanism to promote cold-storage survival. In Aim 3, we will look at combina ons of gene c and environmental factors that promote reanima on of zebrafish embryos from cold storage. Achievement of any one of these aims that result in high viability yields from cold storage can then be developed in Phase II as a genetically integrated line, which can then be broadly commercialized as a product or service to aid researchers in their biobanking efforts with their zebrafish lines.
Public Health Relevance Statement: Narra ve: The zebrafish animal model is a fast-growing biomedical research tool. Yet broader u liza on of zebrafish in research is hampered by an inability to achieve long-term cold storage of transgenic lines. This SBIR project funds the development of systems to enable cryopreserva on of zebrafish lines.
Project Terms: Achievement, Achievement Attainment, Adult, 21+ years old, Adult Human, adulthood, Aftercare, After Care, After-Treatment, post treatment, Animals, Biological Assay, Assay, Bioassay, Biologic Assays, Biomedical Research, Western Blotting, Immunoblotting, Western Immunoblotting, protein blotting, Carrier Proteins, Transport Protein Gene, Transport Proteins, Transporter Protein, Communities, Cryopreservation, Cryofixation, cold preservation, cold storage, Disease, Disorder, Embryo, Embryonic, Embryonic Development, Embryo Development, Embryogenesis, Engineering, Enzyme-Linked Immunosorbent Assay, ELISA, enzyme linked immunoassay, Freezing, Genes, Government, Grant, Growth, Generalized Growth, Tissue Growth, ontogeny, Methods, Mus, Mice, Mice Mammals, Murine, Persons, Names, NIH, National Institutes of Health, United States National Institutes of Health, living system, Organism, Proteins, Reagent, Records, Research, Investigators, Researchers, Research Personnel, Research Resources, Resources, mRNA, Messenger RNA, Sperm, Spermatozoa, zoosperm, sperm cell, Survival Rate, survivorship, Suspension substance, Suspensions, Technology, Testing, Trehalose, Work, Zebrafish, Brachydanio rerio, Danio rerio, Zebra Danio, Zebra Fish, Enhancers, Vitamin D3 Receptor, 1,25-Dihydroxycholecalciferol Receptors, 1,25-Dihydroxyvitamin D 3 Receptors, 1,25-Dihydroxyvitamin D3 Receptors, Calcitriol Receptors, Cholecalciferol Receptors, Vitamin D 3 Receptors, Vitamin D Receptors, Caenorhabditis elegans, C elegans, C. elegans, C.elegans, Acute, Phase, Biological, biologic, Chemicals, Evaluation, Data Bases, data base, Databases, Chaperone, Molecular Chaperones, Funding, Metabolic, Morphology, tool, catalog, Catalogs, Adopted, Hour, Protocol, Protocols documentation, Techniques, Nuclear, Services, Diapause, success, cryogenics, mRNA capping, Animal Models and Related Studies, model of animal, model organism, Animal Model, transgenic, Transgenic Organisms, Structure, disorder model, Disease model, Environmental Factor, environmental risk, Environmental Risk Factor, Coding System, Code, Modeling, protein expression, AFP, FETA, HPAFP, AFP gene, Ectopic Expression, Receptor Signaling, Rodent Model, Small Business Innovation Research Grant, SBIR, Small Business Innovation Research, Monitor, trend, Molecular, Development, developmental, biobank, biorepository, Secure, Systems Development, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR, CRISPR/Cas system, Killifishes, preservation, hormonal signals, hormone signals, antagonist
