The proposal is aimed to develop a high throughput method to study the interactions between chondroitin sulfate (CS) and proteins. CS is a member of glycosaminoglycan family displaying a wide range of biological functions. Glycan Therapeutics is a company specialized in CS and other glycosaminoglycan related research products. In this phase I SBIR proposal, a CS microarray technique will be constructed. The strength of the company is capable of synthesizing structurally defined CS oligosaccharides using the enzymatic method, allowing to synthesize gram-scale of CS oligosaccharide. A single specific aim is proposed in the current application, which to build CS-microarray analysis technique covering 30 different oligosaccharides. Three experiments to support the quality control of the technology are proposed. First experiment is to couple a chemical linker to the synthesized CS oligosaccharides to allow affixation of the oligosaccharides on the array chip. Second experiment is to prepare eight fluorescently labeled CS oligosaccharides to evaluate the efficiency for the affixation to the chip. Third experiment is to identify a CS-binding protein to serve as a positive control for the CS-microarray analysis. In the phase II studies, the number of oligosaccharides array slides will be expanded to 200 to fully represent the structures of CS isolated from natural sources. The success will lead to a convenient approach for improving research in glycosciences.
Public Health Relevance Statement: Project Narrative Chondroitin sulfate is a sulfated polysaccharide displaying a wide range of biological functions. In this project, Glycan Therapeutics plans to develop an innovative chondroitin sulfate microarray technology. The success of the project will provide a new tool to study the interactions between chondroitin sulfate and proteins to advance glycoscience research.
Project Terms: Chondroitin Sulfates, hydrogen sulfate Chondroitin, Communities, Extracellular Matrix, Cell-Extracellular Matrix, ECM, Family, Glycosaminoglycans, Mucopolysaccharides, Grant, Growth, Generalized Growth, Tissue Growth, ontogeny, Lipopolysaccharides, Methods, Metastasis, Metastasize, Metastatic Lesion, Metastatic Mass, Metastatic Neoplasm, Metastatic Tumor, Secondary Neoplasm, Secondary Tumor, cancer metastasis, tumor cell metastasis, Neoplasm Metastasis, Nerve Cells, Nerve Unit, Neural Cell, Neurocyte, neuronal, Neurons, North Carolina, Oligosaccharides, Play, Glycans, Polysaccharides, Proteins, Quality Control, Research, Investigators, Researchers, Research Personnel, chemical structure function, structure function relationship, Structure-Activity Relationship, Technology, Testing, Universities, Generations, Measures, Businesses, Chondroitin Sulfate A, Chondroitin 4 Sulfate, Injury, injuries, base, Organ, Label, improved, Surface, Phase, Biological, biologic, Ensure, Chemicals, Ligand Binding Protein, Ligand Binding Protein Gene, Protein Binding, bound protein, Binding Proteins, Measurement, Funding, Biological Function, Biological Process, Therapeutic, Attenuated, tool, Slide, Source, Pattern, Techniques, System, Sulfate, Services, Molecular Dynamics Simulation, molecular dynamics, success, Structure, member, Reporting, Position, Positioning Attribute, parasite infection, Parasitic infection, response, Molecular Interaction, Binding, Microarray-Based Analysis, microarray analyses, microarray technology, Microarray Analysis, Affinity, Measurable, Small Business Innovation Research Grant, SBIR, Small Business Innovation Research, Preparation, Outcome, Coupling, innovation, innovate, innovative, prototype, phase 1 study, Phase I Study, phase 2 study, phase II study, screening, experimental study, experiment, experimental research
