
TempO-LINC high throughput high sensitivity single cell gene expression profiling assay Ph IIAward last edited on: 3/18/2025
Sponsored Program
SBIRAwarding Agency
NIH : NIGMSTotal Award Amount
$2,902,187Award Phase
2Solicitation Topic Code
859Principal Investigator
Bruce E SeligmannCompany Information
Phase I
Contract Number: 1R43GM140771-01Start Date: 4/15/2021 Completed: 3/31/2022
Phase I year
2021Phase I Amount
$402,266Public Health Relevance Statement:
Project Narrative Singe cell gene expression assays have become important tools to identify functional subtypes of cells and changes in the numbers of cells within each subtype, or of subtypes, resulting from disease and treatments. However, current methods require dedicated hardware and kits, making them expensive, and do not permit low expressed genes that may be key biomarkers to be measured, typically only measuring a limited number of genes/cell, typically not providing quantitative measurements of abundance or changes in expression level, and are often 3' based and so cannot measure splice variants or RNA such as long non-coding RNA that are not polyadenylated. We will demonstrate the feasibility of a novel TempO-LINC assay using a barcoding approach that does not require proprietary hardware, and demonstrate that this assay provides single cell data that measures the low and moderately expressed genes identified from bulk samples, is highly correlated to bulk cell benchmark assay data with respect to the number of genes/cell, represents the dynamic expression range of those genes within each cell, and can provide single cell dose response data - enabling single cell studies not previously possible to understand (e.g.) molecular mechanistic differences at the single cell level of disease, and drug efficacy and safety.
Project Terms:
Archives ; Bar Codes ; barcode ; Biological Assay ; Assay ; Bioassay ; Biologic Assays ; Complementary DNA ; cDNA ; Cell Line ; CellLine ; Strains Cell Lines ; cultured cell line ; Cells ; Cell Body ; Disease ; Disorder ; Gene Expression ; Gene Fusion ; Genes ; Goals ; Human ; Modern Man ; Ligase ; Ligase Gene ; Synthetases ; Ligation ; Closure by Ligation ; Methods ; Mus ; Mice ; Mice Mammals ; Murine ; Noise ; Normal Statistical Distribution ; Gaussian Distribution ; Normal Distribution ; Nucleotides ; Oligonucleotides ; Oligo ; oligos ; Research Personnel ; Investigators ; Researchers ; RNA ; Non-Polyadenylated RNA ; RNA Gene Products ; Ribonucleic Acid ; RNA Splicing ; Splicing ; Signal Transduction ; Cell Communication and Signaling ; Cell Signaling ; Intracellular Communication and Signaling ; Signal Transduction Systems ; Signaling ; biological signal transduction ; Species Specificity ; Specificity ; Sulfhydryl Compounds ; Mercaptans ; Mercapto Compounds ; Thiols ; sulfhydryl group ; Suspensions ; Suspension substance ; Time ; Tissues ; Body Tissues ; Measures ; In Situ Hybridization ; in situ Hybridization Genetics ; in situ Hybridization Staining Method ; base ; crosslink ; cross-link ; detector ; improved ; Phase ; Variant ; Variation ; data quality ; Measurement ; tool ; programs ; Protocol ; Protocols documentation ; In Situ ; Best Practice Analysis ; Benchmarking ; adduct ; Performance ; drug efficacy ; PBMC ; Peripheral Blood Mononuclear Cell ; novel ; sorting ; Sorting - Cell Movement ; Single Base Polymorphism ; single nucleotide variant ; Single Nucleotide Polymorphism ; Gene Expression Monitoring ; Gene Expression Pattern Analysis ; Transcript Expression Analyses ; Transcript Expression Analysis ; gene expression analysis ; gene expression assay ; transcriptional profiling ; Gene Expression Profiling ; Sampling ; response ; drug development ; Functional RNA ; Non-Coding ; Non-Coding RNA ; Non-translated RNA ; Noncoding RNA ; Nontranslated RNA ; noncoding ; Untranslated RNA ; Genomics ; cell preparation ; RNA Polyadenylation ; Polyadenylation ; preventing ; prevent ; CD62L ; HLHRC ; LAM-1 gene ; LECAM1 ; LNHR ; LSEL ; LYAM-1 ; LYAM1 ; Selectin L Gene ; TQ-1 ; SELL gene ; Address ; Dose ; Crosslinker ; Data ; Mouse Cell Line ; Sum ; Cellular Assay ; cell assay ; Transcript ; Molecular ; Process ; Pathway interactions ; pathway ; cost ; Coupling ; innovation ; innovate ; innovative ; combinatorial ; translational medicine ; Biological Markers ; bio-markers ; biologic marker ; biomarker ; transcriptome sequencing ; RNA Seq ; RNA sequencing ; RNAseq ; single cell sequencing ; differential expression ; differentially expressed ; transcriptional differences ; transcriptome ; global gene expression ; global transcription profile ; experimental study ; experiment ; experimental research ; safety assessment ; medication safety ; drug safety ; pharmaceutical safety ;
Phase II
Contract Number: 2R44GM140771-02Start Date: 6/22/2023 Completed: 5/31/2025
Phase II year
2023Phase II Amount
$2,499,921Public Health Relevance Statement:
Project Narrative The analysis of rare and biologically important cell subtypes presents a common challenge in development and disease, where phenotypically variant cell populations can significantly affect the response to therapy and impact patient outcomes. High-throughput single-cell sequencing has proven to be the most successful approach for identifying unique cell subtypes and resolving complex cellular heterogeneity The vision of BioSpyder is to commercially launch a low cost, high-throughput and targeted single-cell multi-omic platform, TempO-LINC, that provides numerous advantages over existing commercial platforms and will enable new studies relying on single-cell sequencing in basic, biopharma, clinical, translational and applied markets.
Project Terms: