Organophosphate (OP) pesticides poisoning is a major environmental and occupational health problem worldwide due to its acute or chronic toxicity. Rapid and accurate detection of personal exposure to OP pesticides is critical to both research and clinical care for victims. However, this detection remains highly variable across commercially available assays, e.g., the Ellman method, and such assays may also not adequately detect low but clinically relevant levels of exposure biomarkers. Moreover, these assays are generally performed in the centralized laboratories, and is slow and expensive. These deficits translate into missed opportunities for the timely initiation of the most appropriate treatment and interventions for acutely or chronically poisoned victims. A simple, rapid, non- or minimally invasive biomonitoring tool for onsite sensitive and accurate detection of personal exposure to OP pesticides is highly desirable and fits the interest of NIEHS exposure program. This STTR phase I project is to develop an innovative integrated smartphone/nanosensor platform for onsite rapid and sensitive detection of OP exposure using a tiny drop of finger-stick blood. This nanosensor will take advantage of nanotechnology and wireless technology and utilize an important biomarker, blood cholinesterase, for the detection of personal OP exposure and the resulted tool has the potential to serve as mHealth for environmental and occupational workers. The research will determine the detection limit, sensitivity, dynamic range, and other key performance metrics of the sensing platform using blood samples in vitro dosed with typical pesticides and validate the platform in field using finger-stick blood samples from farmworkers, and demonstrate the feasibility of the platform for sensitive and accurate detection of exposure to OP pesticides. The resulted nanosensor would be a simple, rapid, cost-effective, and minimally-invasive, point-of-care biomonitoring tool for accurate personal exposure assessment and for on-site rapid detection of exposure to OP pesticides. Thus, this novel sensing platform could serve as a personal meter for self-test just like a glucose meter and could also be used in field, physician's office, bedside, and emergency room. Public Health Relevance Statement Narrative This research project will specifically focus on the development of a novel biomonitoring tool for onsite sensitive and accurate detection of environmental and occupational exposure to OP pesticides and address several important issues of current approaches for pesticide exposure assessment including high cost, inconvenience, low sensitivity and accuracy, inability to analyze and share biomonitoring data.
Project Terms: Agriculture ; agricultural ; Biological Assay ; Assay ; Bioassay ; Biologic Assays ; Biological Monitoring ; Biologic Monitoring ; biomonitoring ; Blood ; Blood Reticuloendothelial System ; Cholinesterases ; Acylcholine acylhydrolase ; Acylcholineacylhydrolase ; choline esterase II ; Data Analyses ; Data Analysis ; data interpretation ; Data Reporting ; data representation ; Cessation of life ; Death ; Diazinon ; Dimpylate ; Disasters ; Chlorpyrifos ; Environmental Health ; Environmental Health Science ; Enzymes ; Enzyme Gene ; Fingers ; Foundations ; Goals ; Health ; Human ; Modern Man ; In Vitro ; Industry ; Insecticides ; Laboratories ; Methods ; Pesticides ; Physicians' Offices ; Poisoning ; poisoned ; Research ; Risk ; Schools ; statistics ; Students ; Technology ; Testing ; Time ; Toxicology ; Translating ; United States ; Work ; Measures ; Occupational Health ; Blood specimen ; Blood Sample ; Site ; Area ; Surface ; Acute ; Chronic ; Phase ; Measurement ; Research Project Grants ; R-Series Research Projects ; R01 Mechanism ; R01 Program ; Research Grants ; Research Projects ; pesticide poisoning ; pesticide toxicity ; Letters ; Exposure to ; farmworker ; farm worker ; tool ; programs ; meter ; Occupational ; interest ; Farm ; farm environment ; Farming environment ; Emergency Department ; Emergency room ; Accident and Emergency department ; data management ; Performance ; Toxicities ; Toxic effect ; novel ; Devices ; Manpower ; personnel ; Human Resources ; organophosphate poisoning ; Sampling ; Property ; response ; glucometer ; glucose meter ; glucose monitor ; develop software ; developing computer software ; software development ; nano tech ; nano technology ; nano-technological ; nanotech ; nanotechnological ; Nanotechnology ; portability ; Intervention Strategies ; interventional strategy ; Intervention ; polyaniline ; Drops ; Cell Phone ; Cellular Telephone ; iPhone ; smart phone ; smartphone ; Cellular Phone ; Buckytubes ; Carbon nano tubes ; Carbon Nanotubes ; Address ; Dose ; Organophosphates ; global health ; Data ; Detection ; Reproducibility ; Environmental and Occupational Exposure ; Epidemiologist ; Small Business Technology Transfer Research ; STTR ; Wireless Technology ; wireless ; Monitor ; Enzyme Inhibition ; Development ; developmental ; point of care ; National Institute of Environmental Health Sciences ; NIEHS ; cost ; rapid detection ; nanowire ; nano wire ; nanosensors ; nano sensing ; nano sensors ; nanosensing ; pesticide exposure ; Treatment Efficacy ; intervention efficacy ; therapeutic efficacy ; therapy efficacy ; cost effective ; Population ; innovation ; innovate ; innovative ; clinically relevant ; clinical relevance ; novel diagnostics ; new diagnostics ; next generation diagnostics ; toxic organophosphate insecticide exposure ; organophosphate exposure ; prototype ; commercialization ; clinical care ; minimally invasive ; Biological Markers ; bio-markers ; biologic marker ; biomarker ; point-of-care diagnostics ; data sharing ; mHealth ; m-Health ; mobile health ; mobile application ; mobile app ; mobile device application ; cloud platform ; cloud server ; acute symptom ; data sharing networks ; data sharing resource ; detection limit ; self testing ; Home ;
