This Small Business Innovation Research Phase I project will demonstrate the feasibility of a novel approach for achieving unprecedented high levels of resolution in ion mobility spectrometry. Our approach will not only improve the resolving power of an ion mobility spectrometer substantially, but also enable efficient separation of ions without the activation of the internal energy of the ions, which is an essential benefit to the study of many life sciences problems. For example, the proposed instrument would be an ideal tool for studying large protein complexes with folded structures vulnerable to even minor alterations from ion internal energy excitation. T here is a hybrid between ion mobility spectrometry and electrophoresis, inheriting the advantages of both methods while expanding the breadth of potential applications. The Phase I focus is on the development of a standalone system that will validate the resolving power and the effective ion temperature during ion mobility analysis. Commercial adoption of this approach will push contemporary ion mobility spectrometry into new frontiers of chemical analysis and will considerably facilitate future applications of âomicsâ methods (i.e., analysis on the systems level) towards clinical use. he innovation proposed
Public Health Relevance Statement: Project Narrative The broader impact of this project is to provide greater capability of high-resolution ion mobility spectrometry into an âomicsâ pipeline (i.e., analysis on the systems level). âOmicsâ-based strategies are already a promising tool for the identification of diagnostic and prognostic biomarkers of disease .
Project Terms: Adoption; Atmosphere; Automobile Driving; base; Biological Process; Biological Sciences; Biology; Buffers; cell analyzer; Cell physiology; Charge; Chemicals; Clinical; combat; Complex; Complex Mixtures; Coupled; Couples; Development; diagnostic biomarker; Disadvantaged; Disease; electric field; Electrophoresis; Elements; frontier; Future; Gases; Heating; Hybrids; Hydrophobicity; improved; Inherited; innovation; instrument; ion mobility; Ions; Knowledge; Liquid Chromatography; Liquid substance; Mass Spectrum Analysis; Measurement; Metabolic Pathway; Methods; Minor; Modeling; Molecular Conformation; Molecular Structure; NMR Spectroscopy; novel strategies; operation; Performance; Phase; preservation; pressure; Process; Prognostic Marker; Protein Analysis; protein complex; Protein Conformation; protein function; protein structure; Proteins; Proteomics; Radial; radio frequency; Research Personnel; Resolution; Signal Transduction; Small Business Innovation Research Grant; Spectrometry; structural biology; Structural Protein; Structure; System; Temperature; Time; tool; Universities; Washington; Width; Work; X-Ray Crys
