Antibody Fragments to Improve HIV gp120 Protein Production
Award last edited on: 6/12/20

Sponsored Program
Awarding Agency
Total Award Amount
Award Phase
Solicitation Topic Code
Principal Investigator
Hiep T Tran

Company Information

Abzyme Therapeutics LLC

321 Jones Boulevard Suite 300
Royersford, PA 19468
   (610) 990-7531
Location: Single
Congr. District: 04
County: Montgomery

Phase I

Contract Number: 75N93019C00009-0-9999-1
Start Date: 00/00/00    Completed: 00/00/00
Phase I year
Phase I Amount
Camelid single domain antibody fragments possess a number of distinctive features including a small size an elevated stability and an ability to recognize epitopes non accessible for conventional antibodiesBesides common research and immunotherapy applications camelid VHHs are also used as co crystallization chaperones protein aggregation controllers and enzyme activity tunersIn this proposal we wish to utilize these unique VHH properties as a chaperone for HIV gp expression folding and stability to improve gp protein productionIn Phase I Camelid VHH antibodies to HIV will be developed that are capable of enhancing gp expression in yeast and in mammalian cells enabling a simple model for rapid and scalable affinity purificationAt least a fold improvement in production in mammalian cell transient expression will be the basis for phase II transition. The effort in Phase II will be to develop stable VHH assisted gp protein production CHO cell lines with significant improvement in yieldGMP manufacturing cell line development and Master Cell bank generation will be performed. Following development scale up of upstream and downstream GMP processes will be completed

Phase II

Contract Number: ----------
Start Date: 00/00/00    Completed: 00/00/00
Phase II year
Phase II Amount