SBIR-STTR Award

Bladder cancer is the sixth most common form of cancer and one of the most deadly. In 2017 alone, approximately 79,000 people will be diagnosed with bladder cancer in the U.S. and 17,000 died of the disease. Patients with advanced or metastatic disease have a dismal prognosis and few treatment options after first-line chemotherapy. More recently, therapy with immune checkpoint inhibitor antibodies has shown durable responses in advanced and metastatic bladder cancer, but the reported response rates warrant improvement. Vaccination against mutant tumor neoepitopes is progressively recognized as a strategy to potentiate the immune response of cells activated through immunological checkpoint blockade. Large-scale sequencing efforts have characterized the mutational landscape in bladder cancer and have already identified commonly mutated genes that could be specifically targeted for cancer therapy. The overall goal of this SBIR phase I proposal is to construct and develop proprietary immunotherapy agents for the treatment of advanced and metastatic bladder cancer based on live Mycobacterium bovis BCG genetically engineered to target mutated forms of FGFR3, an oncogenic driver protein with recurrent alterations in cancer. BCG is a non- pathogenic attenuated intracellular bacterium that stimulates diverse innate and anti-bacterial adaptive immune responses and is well-known for its long safety track record as a tuberculosis vaccine and as a non-specific immune stimulant with modest activity in early-stage bladder cancer. Our hypothesis is that BCG can be successfully modified to express neoantigen protein fragments containing tumor-associated mutations, leading to specific antitumor activity against bladder cancer in vivo. Our first Specific Aim is to construct and validate recombinant BCG strains (rBCG-NEO) that stably express and efficiently secrete proposed tumor neoantigen protein fragments of FGFR3 (FGFR3MUT). Candidate strains with highest secretion and expression levels of human proteins will be selected following the integration of the polyepitope expression plasmids into the BCG genome and prioritized based on their biological properties and functional effects in in vitro immunological assays. The second Specific Aim is to evaluate anti-tumor efficacy of rBCG-FGFR3MUT candidates in a therapeutic vaccination setting. To achieve this, we will employ syngeneic mouse models of bladder cancer in immunocompetent wild-type mice. The most efficacious lead strains will be selected for further preclinical development. In Phase II we plan to test the activity of rBCG-FGFR3MUT in humanized mouse models and evaluate its immunogenicity, safety and additional administration routes. Novel immunotherapy products discovered in this study may be used to treat patients with advanced / metastatic, muscle-invasive bladder cancer or developed for other tumor types such as head-and-neck or lung cancers.

Project Terms:
adaptive immune response; Adult; Anti-Bacterial Agents; antibody inhibitor; Antibody Response; Antigen-Presenting Cells; Antigens; arm; Attenuated; Bacillus (bacterium); Bacillus Calmette-Guerin Therapy; Bacteria; base; BCG Live; BCG Vaccine; Biological; Biotechnology; cancer cell; Cancer Model; cancer type; Cancer Vaccines; Cell surface; Cells; chemotherapy; Clinical; clinical candidate; cost; Dendritic Cells; Development; Diagnosis; Disease; Engineering; experience; Extracellular Domain; FGFR3 gene; Future; Genes; Genetic Engineering; Genome; Goals; Growth; Head Cancer; Human; humanized mouse; Immune; Immune Checkpoint; Immune checkpoint blockade; Immune checkpoint inhibitor; Immune response; Immune system; Immunity; Immunocompetent; immunogenicity; Immunologics; Immunology procedure; Immunotherapeutic agent; Immunotherapy; improved; In Vitro; in vivo; Laboratories; Large-Scale Sequencing; Lead; lead candidate; Life Cycle Stages; Malignant neoplasm of lung; Malignant neoplasm of urinary bladder; Malignant Neoplasms; meetings; Morbidity - disease rate; mortality; mouse model; Mus; Muscle; mutant; Mutate; Mutation; mycobacterial; Mycobacterium bovis; Neck; neoantigens; neoplastic cell; novel; Oncogenes; Oncogenic; outcome forecast; overexpression; Patients; Peptides; Phase; Plasmids; pre-clinical; preclinical development; preclinical study; prevent; Process; Property; protective efficacy; Protein Export Pathway; Protein Fragment; Proteins; rBCG; Recombinants; recruit; Recurrence; Reporting; research clinical testing; response; Route; Safety; screening; Small Business Innovation Research Grant; Solid Neoplasm; synergism; T-Lymphocyte; targeted cancer therapy; Technology; Testing; therapeutic evaluation; therapeutic vaccine; Treatment Efficacy; Tuberculosis Vaccines; tumor; Tumor Antigens; United States National Institutes of Health; Universities; Vaccination; vaccine candidate; vaccine efficacy; Vaccine Therapy; Vaccines; Wild Type Mouse;