?This proposal will assess the quantitative capabilities of surface enhanced Raman scattering (SERS) detection of cocaine and marijuana metabolites in urine samples. Combining low-cost instrumentation and novel chemical sensing provides an innovative new approach for diagnosing illicit drug use. Current methods use a colorimetric indicator to screen specimens for more extensive quantitative analysis in dedicated laboratory facilities. Raman spectroscopy offers a low cost, chemical specific method with potential to determine the concentration of drug related metabolites in biological samples. Ultrasensitive analysis will be achieved using an online SERS flow detector developed in the laboratory of Dr. Z. Schultz at the University of Notre Dame. This flow detector will be evaluated in a Snowy Range Instruments (SnRI) Raman system to determine the necessary requirements for low-cost device that can identify and quantify the presence of drug metabolites comparable to the analysis currently performed in offsite laboratories. Our ultimate goal is a chemical specific, quantitative point-of-care diagnostic that could be produced for $5000. In this phase I project, the Schultz lab will demonstrate the quantitative capabilities of the SERS flow detector for the detection of cocaine and marijuana metabolites. SnRI engineers will incorporate the flow into a commercial Raman system and to determine which components are essential for incorporation in a low cost phase II prototype, appropriate for point-of-care use by non-technical experts. This collaborative research effort will result in new, low cost technology for accurate diagnosis of drug use.
Public Health Relevance Statement: Public Health Relevance: Surface enhanced Raman detection has demonstrated ability as a chemical specific and quantitative method of bioanalysis; however, challenges associated with signal reproducibility and required technical expertise have prevented adoption by non- technical personnel. This feasibility study will explore the combination of low cost Raman technology with recent advances in sampling methods for SERS detection in fluids to enable a low-cost device for assaying for drug metabolites in biological specimens.
NIH Spending Category: Bioengineering; Cannabinoid Research; Drug Abuse (NIDA only); Substance Abuse
Project Terms: accurate diagnosis; addiction; Adoption; base; benzoylecgonine; Biological; Biological Assay; Biological Models; Businesses; Cannabis; Chemicals; Chromatography; Cocaine; commercialization; Complex Mixtures; cost; Cost Analysis; Coupled; Detection; detector; Development; Devices; Diagnosis; Diagnostic; Doctor of Medicine; Drug abuse; drug of abuse; Drug usage; Engineering; Exhibits; Feasibility Studies; Forensic Medicine; Future; Gases; Goals; Human Resources; Illicit Drug Detections; Illicit Drugs; improved; innovation; instrument; instrumentation; Isomerism; Laboratories; laboratory facility; Liquid substance; Marijuana; mass spectrometer; Mass Spectrum Analysis; Metabolic; Methods; novel; novel strategies; Nuclear Magnetic Resonance; Pattern; Pharmaceutical Preparations; Phase; Play; point of care; point-of-care diagnostics; prevent; Process; prototype; public health relevance; Raman Spectrum Analysis; reconstitution; Regression Analysis; Relative (related person); Reproducibility; Research; residence; Resolution; Sampling; Signal Transduction; Specimen; Surface; System; Technical Expertise; Technology; Tetrahydrocannabinol; Time; Universities; Urine
