SBIR-STTR Award

Our goal in this project is to develop a uniform method for assembling large DNA molecules by ligation of sticky-ended fragments, regardless of the sequence, the fragnent length, or the presence of restriction enzyme sites. If we are successful, this techndogy could be used to assemble full-length cDNAs, mammalian genes, knockout contstructs, gene clusters, and even whole BACs or bacterial genomes. The combination of a universal fragment assembly technology with Blue Heron's core technology, gene synthesis, will provide researchers with rapid, cost-effective access to any DNA sequence, regardless of size or sequence composition. It will speed the pace of research by allowing scientists to focus on experiments rather than cuttingand pasting DNA molecules. It will also enable new projects that would be impossible or impractically expensive without full control of large DNA sequences.

Thesaurus Terms:
DNA, DNA methylation, genetic technique, molecular cloning, nucleic acid sequence, synthetic nucleic acid, technology /technique development DNA binding protein, nucleic acid chemical synthesis, nucleic acid purification, restriction endonuclease biotechnology, high throughput technology, transfection /expression vector

Our goal in this project is to develop a uniform method for assembling large DNA molecules by ligation of sticky-ended fragments, regardless of the sequence, the fragment length, or the presence of restriction enzyme sites. If we are successful, this technology could be used to assemble full-length cDNAs, mammalian genes, knockout constructs, gene clusters, and even whole BACs or bacterial genomes. The combination of a universal fragment assembly technology with Blue Heron's core technology, gene synthesis, will provide researchers with rapid, cost-effective access to any DNA sequence, regardless of size or sequence composition. It will speed the pace of research by allowing scientists to focus on experiments rather than cutting and pasting DNA molecules. It wll also enable new projects that would be impossible or impractically expensive without full control of large DNA sequences.

Thesaurus Terms:
DNA, DNA methylation, expression cloning, macromolecule, method development, molecular assembly /self assembly, nucleic acid chemical synthesis DNA binding protein, binding site, methyltransferase artificial chromosome, biotechnology, high throughput technology, plasmid, restriction endonuclease, transfection /expression vector