Syntheses and/or evaluation of health, physiological, and environmental standards, drugs, and other chemicals are often not as problematic as the purification of the target compounds from the crude reaction mixtures' by- products, side-products or hydrolysis/oxidation products of very similar structural composition. Low temperature glassy carbon (LTGC) HPLC phases offer a potentially unique approach to separating these intricate mixtures. The glassy carbon structure is known to have remarkable ability in separating samples based on their size and shape. Furthermore, the separation characteristics of the LTGC phase can be altered or "tuned" into producing a desired separation simply by varying the Polymerization.coating temperature. The goal of this proposal is to: 1) synthesize a large batch of LTCG polymer precursor, 2) develop a coating procedure(s) for porous and solid silica and zirconia particles, 3) further treat the coated phase at higher temperatures (400 degrees C, 600 degrees C, and 800 degrees C) to "tune" in desirable separation characteristics, and 4) probe the utility of the resulting HPLC phase as a commercial product for the isolation of a geometrical isomer PAH mixture, a cresol mixture, and racemic 3,5-dinitrobenzoyl-1- phenethylamine. The potential market value for LTGC HPLC columns has been estimated to be $10,000,000.00 per year.Proposed commercial application:Potential commercial applications for LTGC HPLC columns include PAH's, PCB's, carcinogens chiral compounds, drugs and their metabolites, synthetic intermediates, pesticides and herbicides, petroleum constituents and food and beverage chemicals.National Institute fo General Medical Sciences (NIGMS)
