The objective is to investigate the saliva's potential as a biological medium that can be non-invasively obtained for the screening of elevated lead levels in humans. The goals of the feasibility study are threefold: (1) to select a convenient method of specimen collection; (2) to adapt an analytical method to measure the concentrations of lead expected in the specimen; and (3) to determine the correlation of lead in blood and saliva from simultaneously collected specimens. Saliva will be collected either as an ultrafiltrate collected in the mouth with an osmotic device, by means of a special swab, or as whole saliva either expelled directly or collected with a pipette. The concentration of lead in saliva will be measured by graphite furnace atomic absorption spectroscopy (AAS) with palladium as a modifier. Lead concentrations in saliva are normally lower than concentrations in blood. Therefore, the sensitivity of the AAS method will be enhanced 50 to 100-fold to make the reliable measurement of lead in saliva possible. Finally, matched samples of blood and saliva will be collected from individuals whose lead exposures are different, and the concentrations will be correlated. These investigations will provide the groundwork for a comprehensive study, performed in Phase II, that results in the recommendation of threshold levels for lead in saliva as specimen.Awardee's statement of the potential commercial applications of the research:In the United States, we estimate that 17 million screening tests for elevated lead levels will be performed annually on children under three years of age. The follow-up screening of children with elevated lead levels could increase this number. Use of saliva specimens will permit commercialization of the appropriate collection devices and testing services in specialized laboratories serving large geographical areas.National Institute of Child Health and Human Development (NICHD)
