SBIR-STTR Award

Skin cancer, resulting from ultraviolet (UV) light damage to DNA, has increased dramatically in the United States and throughout the world. Applied Genetics, Inc , has developed a method to enhance DNA repair in human cells by treatment with liposomes containing W-DNA repair enzymes. Phase I research will measure mutation frequency in human cells treated with liposomes to determine if enhanced DNA repair will prevent mutations that lead to cancer. The patch size of repaired regions will also be measured, and the repair of 6-4 photoproducts in DNA will be measured. This characterization of enhanced DNA repair will answer some basic science questions and is important before beginning animal testing ofliposomes in Phase II. The goal is a topical lotion containing DNA repair liposomes to prevent skin cancer after W exposureAwardee's statement of the potential commercial applications of the research:Liposomes containing DNA repair enzymes will be added to skin care products, including sun lotions, cosmetics, and pharmaceutical products. There is substantial interest among these manufacturers for new skin care products relating to sun exposure, particularly for treatment of diseases related to defects in DNA repairNational Cancer Institute (NCI)

Skin cancer has reached epidemic proportion in the U. S., and Applied Genetics has developed T4N5 liposomes for topical application to prevent skin cancer induced by sun exposure. The liposomes encapsulate T4 endonuclease V, an enzyme which initiates removal of UV damaged DNA, stimulates repair and prevents cell death and mutations. Phase II research will focus on the physical characterization and stabilization of the liposomes, the penetration of human skin, and animal studies on UV induction of skin cancer and alteration of the immune response. This work will contribute to the development of a product for treatment of human disease predisposing of skin cancer.Awardee's statement of the potential commercial applications of the research:This research will contribute to the development of a practical form of liposome encapsulated DNA repair enzymes for topical administration to prevent human skin cancer from sun exposure.National Cancer Institute (NCI)