SBIR-STTR Award

The role of Eikenella corrodens, Fusobacterium nucleatum, and Wolinella recta as pathogens associated in destructive periodontal disease has been described in a number of studies using culture based assay systems. The standard culture methods are inadequate for the routine diagnosis of these pathogens. By developing DNA probe-based assays for the detection of thes pathogens, it would be practical to deliver diagnostically valuable information to the dental practitioner on a routine basis. DNA probes for the detection of target DNA from these organisms will be developed by isolation of whole genomic sequences or species- specific segments and use in a standard membrane hybridization assay. Conventional radio-labelling, novel sample preparation and hybridization, and recombinant DNA technology will be employed. Phase II objectives will involve clinical studies and non-isotopic methodology. The long term objective is to provide a rapid, sensitive and accurate diagnostic battery of DNA probes for the detection of periodontal disease for commercial application.National Institute of Dental Research (NIDR)

___(NOTE: Note: no official Abstract exists of this Phase II projects. Abstract is modified by idi from relevant Phase I data. The specific Phase II work statement and objectives may differ)___ The role of Eikenella corrodens, Fusobacterium nucleatum, and Wolinella recta as pathogens associated in destructive periodontal disease has been described in a number of studies using culture based assay systems. The standard culture methods are inadequate for the routine diagnosis of these pathogens. By developing DNA probe-based assays for the detection of thes pathogens, it would be practical to deliver diagnostically valuable information to the dental practitioner on a routine basis. DNA probes for the detection of target DNA from these organisms will be developed by isolation of whole genomic sequences or species- specific segments and use in a standard membrane hybridization assay. Conventional radio-labelling, novel sample preparation and hybridization, and recombinant DNA technology will be employed. Phase II objectives will involve clinical studies and non-isotopic methodology. The long term objective is to provide a rapid, sensitive and accurate diagnostic battery of DNA probes for the detection of periodontal disease for commercial application.National Institute of Dental Research (NIDR)

Project Terms:
Actinobacillus actinomycetemcomitans; Bacteroidaceae; Bacteroides; Bacteroides gingivalis; dental disorder diagnosis; dental plaque; diagnosis design /evaluation; diagnosis quality /standard; Fusobacterium nucleatum; genetic library; gram negative bacteria; human subject; longitudinal human study; microorganism culture; molecular cloning; nucleic acid hybridization; nucleic acid probes; oral bacteria; periodontium disorder; prognosis; recombinant DNA; tooth loss