SBIR-STTR Award

BIOTIX proposes to develop systems for stably cloning small and large DNA fragments that are also optimized for producing a series of overlapping subclones using either the singlestranded or the double-stranded procedure. The vectors will be constructed from existing vectors utilizing a powerful new insertion technique. Success in the construction of these vector systems will lead to the development and production of a series of integrated kits during Phase II. The specific Phase I objectives are: (1) construction of a vector with a multiple cloning region optimized for both single- and double stranded subcloning procedures; and (2) modification of the vector to include a cos site to permit up to 40 kb fragments to be stably cloned.National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)