The overall objective of this project is to produce a panel of polyclonal (goat) antibodies against colorectal cancer markers such as colon-specific antigen protein (CSA colon-ovary tumor antigen (COTA), and carcinoembryonic antigen (CF.A) for t @e development of serodiagnostic and immunohistochemical reagents. These reagents will eventually be made into kits for commercial use. Phase I of this project is emphasizing only the immunohistochemical application of these reagents. The immunological approach to early detection and diagnosis of cancer relies on the premise that tumor specific markers achieve circulation in the blood and are expressed both internally and on the surface of tumors in cancer patients. Immunodetection of these markers in our laboratory and others supports this premise.In Phase 1, polygonal antibodies to CSA,), COTA, and CEA are being produced; the specificity of these antibodies is ensured by a series of affinity adsorption procedures. Preliminary histological testing of this antibody panel will occur in New Mexico and in San Diego, CA. In Phase 11, production of monoclonal antibodies (murine/human) toward CSADI COTA, and CEA will begin and the F(abl)2 fragments will be prepared under Food 'and Drug Administration Good Laboratory Practice/Good Manufacturing Practice (GLP/GMP) guidelines. While these later products may also prove useful as serodiagnostics and immunohistochemical reagents, their possible use in radioimmunoimaging will be evaluated.
Thesaurus Terms: Genetic Mapping, Genetic Markers, Immunology, Antibodies, Immunology, Antigens Carcinoembryonal, Neoplasms Diagnosis, Immunodiagnosis Of Neoplasms, Neoplasms Immunology, Tumor Antigens, Neoplasms Of Gastrointestinal System, Colon Growths, Neoplasms Of Gastrointestinal System, Rectum Growths, Neoplasms Of Reproductive System Female, Ovary Neoplasms, Surgery, Anesthesiology And Trauma Study Section Diagnostic Quality-Standards, Diagnostic Tests, Early Diagnosis, Immunological Tests And Immunoassay, Enzyme-Linked Immunosorbent Assay (Elisa), Immunological Tests And Immunoassay, Immunofluorescence, Immunological Tests And Immunoassay, Radioimmunoassay, Immunology, Antibody Specificity, Immunology, Antigens, Surface Antigens (General), Immunology, Serology, Neoplastic Growth, Neoplasms Metastasis, Immunochemistry Human, Tissues, Fluids Etc. From Non-Related Sources Outside Immediate Project, Mammals, Artiodactyla, Goats National Cancer Institute
