This project is developing a self-contained, solid- phase, colorimetric immunoassay for the rapid identification of biological analytes for use in a micro-gravity environment. The patented technology for this simple-to-use, non-instrumented device can detect as few as 2 x 103 Group A streptococci in less than 5 minutes. For use in space, the design concept is a single-use tube which has a dacron swab for sample collection, a series of chambers containing lyophilized extraction and colloidal gold immuno-reagents, a reconstitution buffer, and a reaction zone on a fixed membrane. After using the swab to collect a biological specimen, it is returned to the device and pushed through the reagent chambers. After one to five minutes, the swab is pushed further into the tube device where the reactants diffuse for at least one minute into a capture membrane. The membrane is then observed with the naked eye for the presence of an analyte indicated by development of a red spot. This device is ideal for use in space: all transfer of reactants is accomplished by movement of the swab and diffusion; no washing, additional steps, or reagents are required; each single-use test can be stored at room temperature; and the device is designed for use by untrained personnel.
Potential Commercial Applications: This technology can be utilized for diagnostic purposes by untrained personnel for physicians offices, the home, third world countries, space travel, the military and environmental testing.STATUS: Project Proceded to Phase II
