This project will prepare probes suitable for fractionating the centromere region of the maize genome, and then confirmed centromere clones will be used to construct a maize mini-chromosome. These mini-chromosomes will remain distinct from the host genome and will allow the delivery of very large DNA segments, including those that encode all of the genes in a complex pathway. In Phase I, DNA from maize seedlings will be harvested and DNA fragments containing centromere-specific modifications or centromere-specific repeats will be purified. These fragments will be cloned, sequenced, and then used as probes for hybridization to BAC clones. In Phase II, these BAC clones will be combined with other chromosome components to yield mini-chromosomes. Commercial Applications And Other Benefits as described by awardee: The technology should impact agricultural biotechnology by enabling the transfer of multiple genes into a plant. Other applications include synthesis of biomaterials and pharmaceuticals, plants with improved yield and nutritional value, and environmentally friendly specialty chemicals.
