The objective of the study is to define conditions for rapidly dissociating antigen, hapten, bacteria and virus from solid surface bound antibody without altering antibody affinity or specificity so that regenerated surfaces can be used repeatedly to specifically capture and detect antigen or hapten. Polyclonal and monoclonal antibody (igg, f(ab)2 and fab fragments) will be covalently bound to solid surfaces including glass beads, polystyrene beads, immobilon and immunodyne membranes and polystyrene microtiter plates. Tests will be done to determine how well antibody is covalently bound to the solid surface and how well antigen is dissociated from the bound antibody. Model systems will be used to examine dissociation of soluble antigen, hapten and insoluble antigen from covalently bound antibody. The model system for studying dissociation of soluble antigen from bound antibody will be the antihuman igg system used in the phase i effort. The model system for studying dissociation of hapten from bound antibody will be theophylline and fluorescein and their respective antibodies. The model system for studying dissociation of antibody-insoluble antigen complexes will use e. Coli and hiv and their respective antibodies.
