Intrinsic remediation is an increasingly acceptable alternative to costly engineered remediation of contaminated sites that pose acceptable risk. One factor affecting intrinsic remediation potential is the presence of biologically available terminal electron acceptors for aerobic and anaerobic biodegradation. Phase I demonstrated the feasibility of a bioassay for iron bioavailable to iron-reducing bacteria during aromatic hydrocarbon oxidation. The AFCEE intrinsic remediation protocol considers additional factors including degradation rate. In support of cost-effective and systematic implementation of the protocol, the test kit will quantify compound-specific degradation rates and bioavailable electron acceptors in methanogenic, sulfidogenic, iron-reducing, denitrifying and aerobic environments. These environments will be identified using a novel and inexpensive H2 assay. Software will process kit data and existing site data and report a qualitative (e.g., consistency with future land use) and quantitative (e.g., biodegradation rate, plume migration) intrinsic remediation assessment. Phase II involves optimization, packaging, field validation, and delivery of a prototype for fuel hydrocarbon sites. With limited modifications, the kit may be utilized for intrinsic bioremediation of other contaminants such as trichloroethylene (TCE). Market research demonstrated a demand for these kits; commercialization plans are in progress with a committed Phase II/III partner
