DNA and protein chip microarray technology relies upon fluorescent labels to visualize DNA hybridization and antigen/antibody binding. A recent breakthrough at Oak Ridge National Laboratory has demonstrated the possibility of label-free detection of DNA hybridization using a microcantilever as the "chip reader." A label-free analyte sensing system would: (1) solve the problem with photobleaching and spectral overlap associated with fluorescent dyes; (2) be cost effective (less labor and expendables required) and potentially; and (3) could provide increased sensitivity compared to existing protein chip technologies with potential femtogram (attomolar) level quantification. However, proof-of-concept data is required in order to demonstrate that this novel microcantilever-based reader will have utility in sensing antigen/antibody binding. This project will apply the microcantilever-based "chip reader" to a high throughput setting, with potential utility in research as a novel proteomic tool and/or medical diagnostic device. Phase I will determine proof-of-concept by showing that two different antigen/antibody combinations can be detected with this system. The study will utilize the head of an Atomic Force Microscope, with its associated microcantilever positioned to function as a "chip reader". Phase II focus will develop a prototype device that functions as the chip reader, based on the Atomic Force Microscope design but with significantly less production cost.
Commercial Applications and Other Benefits as described by the awardee: A label-free, high-throughput device should potentially provide femtogram level sensitivity as well as a cost-effective alternative to the confocal laser/fluorescent dye system that exists in the current protein chip industry. Commercial applications will be focused on the research and medical diagnostic communities. The market strategy would involve sales of selling the microcantilever-based "reader" along with a recurring supply of protein chips. The long-term market for this device and chip sales is the $11 Billion In Vitro Diagnostics industry. Sales of a microcantilever-based diagnostic chip reader are projected to exceed $100 million by 2011
