Bioremediation Consulting Inc. (BCI) provides microbiological and analytical services for environmental and engineering companies working with chlorinated solvents and other recalcitrant chemicals such as 1,4-dioxane. They also address petroleum and coal tar remediation. Their services include microcosm and treatability testing, laboratory investigation of natural attenuation, support for site remediation and field monitoring, product testing, and development of bacterial cultures for bioaugmentation. Their anaerobic cultures include several strains of Dehalococcoides, as well as cultures that degrade 1,1,1-TCA, dichloromethane, and toluene. BCI specializes in site-specific design of microcosm tests constructed from site soil and ground water. These microcosms are monitored by BCI to determine the extent and nature of natural attenuation processes, as well as the potential for enhanced in situ bioremediation. They have also developed specialized techniques for the manipulation of a wide variety of microorganisms under both aerobic and anaerobic conditions, and the capability for evaluating their contribution to contaminant biodegradation. The BCI laboratory has expertise in the analysis of: Natural Attenuation Parameters, dissolved gases such as methane, ethene, and ethane by gas chromatography, volatile chlorinated compounds GC screens, alcohols, glycols and water soluble ethers by gas chromatography, organic acids such as formate, lactate, and acetate by capillary ion electrophoresis and by ion chromatography, molecular hydrogen by reduction gas analysis, PCR based identification of environmentally active microbial species such as Dehalococcoides ethenogenes, microbiological enumeration of total heterotrophs and specific chemical degrading bacteria.