(Adapted from Applicant's Abstract): The goal of this proposal is to create a simple, accurate and low cost method for drug susceptibility profiling HIV-positive patients. It is proposed to use linear signal amplification to rapidly detect unique nucleic acid sequences that may be present as a small fraction (<0.1 - 10%) of the virus population. The PI contends that this makes the method well suited for detection of targeted mutations in the variable genetic background characteristic of HIV. This phase I study describes detection of 4 highly prevalent HIV mutations in the pol gene that reduce susceptibility to widely used reverse transcriptase inhibitors. The proposal includes plans to validate the methodology on clinical samples.Proposed Commercial Application:Not avaliable
Thesaurus Terms:AIDS /HIV test, HIV infection, antiAIDS agent, drug metabolism, drug screening /evaluation, gene expression biotechnology, gene mutation, health care cost /financing, method development, nucleic acid sequence, virus genetics