SBIR-STTR Award

Isolation and purificationof Michellamine B
Award last edited on: 4/16/02

Sponsored Program
SBIR
Awarding Agency
NIH : NCI
Total Award Amount
$72,150
Award Phase
1
Solicitation Topic Code
-----

Principal Investigator
M Lalith Kumar

Company Information

Thar Technologies Inc (AKA: Thar Designs Inc)

730 William Pitt Way
Pittsburgh, PA 15238
   (412) 826-3939
   murray@thardesigns.com
   www.thartech.com
Location: Multiple
Congr. District: 17
County: Allegheny

Phase I

Contract Number: 1R43CA065381-01
Start Date: 00/00/00    Completed: 00/00/00
Phase I year
1994
Phase I Amount
$72,150
We will use supercritical fluid extraction (SFE) to extract michellamine from the plant material Anistrocladus korupensis. We will also separate the monomers and the dimers by manipulating the pressure and temperature of the extraction. To separate michellamin A from B, we have two unique solutions that have not been tried earlier. One would be using a unique stereospecific probe and the other is to use a chromatographic technique. We believe that at least one of them would work for sure.Commercial ApplicationsThis project will result in a number of benefits to the natural product industry. However, the most important aspect is the separation of michellamine B from other naphthyl tetrahydroisoquinoline alkaloid dimers such as michellamine A using either SFE with a stereochemical probe modifier or using prep SFC. Other potential benefits of this technique are the purity of the michellamine B as compared to that produced through prep HPLC and the elimination of large amount of hydrocarbon solvents. These solvents are not only expensive to use and dispose, but EPA has classified many solvents as carcinogenic materials. EPA wants the industry to eliminate them and find alternate methods to process natural products.National Cancer Institute (NCI)

Phase II

Contract Number: ----------
Start Date: 00/00/00    Completed: 00/00/00
Phase II year
----
Phase II Amount
----