This research project will provide a new and improved way in which researchers can treat preparations of total ribonucleic acid (RNA) to obtain messenger RNA (mRNA), free of ribosomal RNA (rRNA). The traditional method has been to capture the MRNA, which has long repeats of adenines (A) at its 3' end, by using immobilized oligo (dT) (16 to 30 thymidines long), and then to wash away the RRNA which does not bind. This work will provide a way to remove RRNA from RNA preparations, leaving the MRNA. This will be done by passing the RNA preparations over beads that have oligonucleotides (short single stranded deoxynucleotide sequences) attached to them which are complementary to conserved sequences of RRNA. In this way the RRNA will be bound and the MRNA will flow through. This new method will be attractive to researchers since it provides a better way to obtain MRNA. First, the yields of MRNA should be higher since even MRNA with short poly A tails will be isolated. Secondly, it gives researchers access to non polyadenylated mRNA's which are lost in the oligo (dT) column method.Awardee's statement of the potential commercial applications of the research:Researchers in private industry and academia, who work with MRNA, need a way to remove the contaminating RRNA. This research would provide a simple, effective way to do this.National Institute of General Medical Sciences (NIGMS)
