Current methodology for the detection of multiple residues of organophosphates requires sample cleanup followed by either separation using sophisticated analytical instrumentation or an assay in one of the cholinesterase enzyme systems, which has the disadvantage of poor stability. The advantages of reagent stability, specificity, and sensitivity offered by immunoassay technology can be applied to the multiresidue detection of organophosphates if antibodies with broad recognition for a characteristic structural feature are produced. If the feasibility of this approach is demonstrated, it would have broad applicability to the detection of other groups of related pesticides. Development of broad screening systems would significantly reduce the cost of testing. Such systems would also be useful in the protection of human health and safety as early detection of these toxic substances can help to minimize personal exposure.
Anticipated Results:Rapid, easy-to-use field tests for the detection of multiple residues of organophosphates would provide useful screening kits to determine water, soil, or food quality. The availability of products would permit individuals to test well water, workers to test areas that have been recently treated, or food chains to test produce. All of these applications would help to minimize personal exposure.National Institute Of Environmental Health Sciences (NIEHS)
