This research proposal attempts to develop oral delivery systems for vaccine and vaccines to fish as an effective alternative to current vaccination methods such as intraperitoneal injection and immersion vaccination. It deals with basic biochemical and physiologic determinants for their oral delivery. Its specific aim is to examine factors that affect the Iymphatic uptake of macromolecular therapeutic agents from the gastrointestinal (GI) tract so an immunologically intact, therapeutically competent vaccine, Vibrio anguillarum. can be administered orally to rainbow trout, Oncorhvnchus mvkiss. In this research, we propose to deliver modified vaccine to the Iymphatic system via oral administration by mimicking the Iysophosphatidylcholine pathway of fat absorption in fish.Applications:Successful development of oral vaccine against vibrosis will encourage and induce the development of other oral vaccines for fish. As a result, the loss in fish due to stress from being handled and the interruption of husbandry routine resulting from mass vaccination on fish will be reduced to a minimum.
