The development of new efficient tools for measuring electroencephalogram (EEG) in freely moving mice is highly important in both academic and commercial research studies. EEG provides valuable information on the impact of drugs on arousal and sleep architecture, and allows for the assessment of their neuropharmacological characteristics in vivo, including central exposure and information on kinetics. It is particularly useful for the development of novel medications in the animal models of neurodegenerative diseases because these models are characterized by specific changes in the EEG pattern that gradually develop over the course of the disease. Despite EEG measurements being highly desirable in many research studies, their use is currently limited due to technical difficulties associated with time-consuming surgeries required for implanting EEG electrodes (i.e., more than a half an hour per one surgery) and a high cost of surgical materials. We recently filed a provisional patent application describing a new system and procedure for the implantation of EEG electrodes in mice that can be performed within a few minutes. This procedure is considered non-surgical because it is minimally invasive and does not involve cutting. In this project, will enhance the key features of the system to enable a larger set of research applications and start disseminating our products in research laboratories. Our ultimate goal is to provide user-friendly tools for recording and analyzing EEG in freely moving mice that will both benefit the welfare of research animals and allow neuroscientists completing research projects faster and with a lower cost.
Public Health Relevance Statement: PROJECT NARRATIVE The impetus of this proposal is the development of a new system that allows non-surgical placement of EEG electrodes in mice. Anticipated results from our easy-to-use system will allow for more consistent data due to reduced data artifacts and less stress induced on the mice, provide the ability for more normal activity patterns to be conducted by the mice, allow high-throughput studies, and improve the reliability and accuracy of drug generation.
Project Terms: ages; Age; Animals; Anxiety; Arousal; Behavior; Animal Behavior; bone; Disease; Disorder; Pharmaceutical Preparations; Drugs; Medication; Pharmaceutic Preparations; drug/agent; Electrodes; Electroencephalography; EEG; Electroencephalogram; Engineering; Fright; Fear; Fracture; bone fracture; Goals; Head; Implantation procedure; implant placement; implant procedure; Kinetics; Laboratories; Laboratory Research; Locomotion; Memory; Methods; Mission; Mus; Mice; Mice Mammals; Murine; Legal patent; Patents; pressure; Research; Research Personnel; Investigators; Researchers; Silicone Elastomers; Silicone Rubber; cranium; Skull; Sleep; Sleep Deprivation; deficient sleep; inadequate sleep; insufficient sleep; sleep debt; sleep deficiency; sleep deficit; sleep insufficiency; sleep loss; Stress; Telemetry; Telemetries; telemetric; Testing; Thinness; Leanness; Time; Translations; translation; Work Simplification; Writing; Generations; Measures; Artifacts; Morphologic artifacts; Businesses; animal welfare research; improved; Procedures; Penetration; Phase; Pythons; Measurement; R-Series Research Projects; R01 Mechanism; R01 Program; Research Grants; Research Projects; Research Project Grants; tool; programs; Hour; Pattern; System; Neurodegenerative Disorders; Degenerative Neurologic Diseases; Degenerative Neurologic Disorders; Nervous System Degenerative Diseases; Neural Degenerative Diseases; Neural degenerative Disorders; Neurodegenerative Diseases; Neurologic Degenerative Conditions; degenerative diseases of motor and sensory neurons; degenerative neurological diseases; neurodegenerative illness; Operative Surgical Procedures; Operative Procedures; Surgical; Surgical Interventions; Surgical Procedure; surgery; surgery material; surgical material; experience; Animal Model; Animal Models and Related Studies; model of animal; Biosensor; biological sensor; novel; research study; new technology; novel technologies; drinking; Modeling; Data; in vivo; Monitor; Characteristics; Development; developmental; cost; feeding; designing; design; Sleep Architecture; Consumption; Implant; user-friendly; implantation; murine model; mouse model; commercialization; minimally invasive; data reduction; Brain Research through Advancing Innovative Neurotechnologies initiative; BRAIN initiative; wireless; miniaturized device; device miniaturization; miniaturized electronics; miniaturized technologies
