SBIR-STTR Award

Direct to Phase II

Measuring the kinetics of small molecule binding to protein receptors and biochemical reactions of proteins, suchas post-translational protein modifications, is a basic task in the understanding of diseases, discovering ofdiagnosis biomarkers, and screening of drugs. Various label-free techniques have been developed, but theirsensitivity decreases with the molecular mass, which makes it challenging to detect small molecules andbiochemical reactions. A charge sensitive optical detection (CSOD) system is proposed to address this unmetneed. The technology is not only sensitive to small molecules, but also compatible with the standard microplatetechnology, which is particularly suitable for high-throughput applications. The working principle was establishedand validated with the support of NIH NCI IMAT (Innovative Molecular Analysis Technologies for cancerresearch) grants (R21 and R33) to the Arizona State University (ASU) team. In this SBIR direct phase II project,Biosensing Instrument Inc. (BI) will work with the ASU team to develop a commercially viable prototype system.We will continue collaborations with potential customers in biomedical research and pharmaceutical industriesfor testing samples relevant to their interest and validating the performance usability of the developing system. The success of the project will lead to a new high-throughput screening technology for measuring molecularinteractions, particularly small molecule interactions with proteins, and post-translational modifications ofproteins. These processes are highly important for biomarker discovery, disease diagnosis and drug screening.

Public Health Relevance Statement:
PROJECT NARRATIVE A charge sensitive optical detection technology will be developed for label-free quantification of small molecule- protein interactions, and biochemical reactions of proteins. This capability will have a large impact on biomedical research of cellular processes, discovery of biomarkers, and screening of drug candidates.

Project Terms:
Adoption ; Arizona ; Biomedical Research ; Biosensing Techniques ; Biosensing Technics ; biosensing ; Buffers ; Cell physiology ; Cell Function ; Cell Process ; Cellular Function ; Cellular Physiology ; Cellular Process ; Subcellular Process ; Charge ; Data Analyses ; Data Analysis ; data interpretation ; Diagnosis ; Disease ; Disorder ; Drug Industry ; Pharmaceutic Industry ; Pharmaceutical Industry ; Pharmaceutical Preparations ; Drugs ; Medication ; Pharmaceutic Preparations ; drug/agent ; Electrons ; Negative Beta Particle ; Negatrons ; Feedback ; Kinetics ; Membrane Proteins ; Membrane Protein Gene ; Membrane-Associated Proteins ; Surface Proteins ; Molecular Weight ; United States National Institutes of Health ; NIH ; National Institutes of Health ; Noise ; Optics ; optical ; Peptides ; Phosphorylation ; Protein Phosphorylation ; Post-Translational Protein Processing ; Post-Translational Modification Protein/Amino Acid Biochemistry ; Post-Translational Modifications ; Post-Translational Protein Modification ; Posttranslational Modifications ; Posttranslational Protein Processing ; Protein Modification ; Proteins ; Reading ; research and development ; Development and Research ; R & D ; R&D ; Signal Transduction ; Cell Communication and Signaling ; Cell Signaling ; Intracellular Communication and Signaling ; Signal Transduction Systems ; Signaling ; biological signal transduction ; Computer software ; Software ; Technology ; Testing ; Time ; Universities ; Work ; Measures ; Label ; sensor ; Procedures ; Phase ; Fiber ; Serum ; Blood Serum ; Measurement ; Research Project Grants ; R-Series Research Projects ; R01 Mechanism ; R01 Program ; Research Grants ; Research Projects ; Collaborations ; electric field ; instrument ; Peptide Library ; mechanical ; Mechanics ; Reaction ; Techniques ; System ; interest ; Performance ; Receptor Protein ; receptor ; success ; molecular mass ; Modeling ; Sampling ; drug development ; High Throughput Assay ; high throughput screening ; drug discovery ; Molecular Interaction ; Binding ; data processing ; computerized data processing ; small molecule ; Address ; Affinity ; Enzymatic Reaction ; Biochemical Reaction ; Detection ; Molecular Analysis ; Reader ; Phosphorylation Inhibition ; Small Business Innovation Research Grant ; SBIR ; Small Business Innovation Research ; Validation ; Molecular ; Process ; Development ; developmental ; cost ; anticancer research ; anti-cancer research ; cancer research ; design ; designing ; novel strategies ; new approaches ; novel approaches ; novel strategy ; cost effective ; innovation ; innovate ; innovative ; usability ; optical fiber ; prototype ; commercialization ; real world application ; Biological Markers ; bio-markers ; biologic marker ; biomarker ; disease diagnosis ; drug candidate ; screening ; Drug Targeting ; Geometry ; biomarker discovery ; Drug Screening ; detection limit ; detection method ; detection procedure ; detection technique ; detection platform ; detection system ;