Irreproducibility of research data due to misidentification or propagated errors within cell lines impacts $3.7bn research funding in the US each year. Moreover, it is not easy to rapidly and inexpensively monitor the genomic composition and evolution of a cell line during its long-term culture. The project concerns development of a technology that integrates extraction of DNA from in vitro cultured cell lines with single molecule analysis of the genomes present in the population of cells. Phase 1 of the project will demonstrate the feasibility of acquiring sequence, structural and epigenomic information robust enough to identify and characterize cell lines.
Public Health Relevance Statement: Narrative A challenge for researchers is identifying subtle changes in the genome of cells that are used for research studies, as these alterations can change the outcome of otherwise identical experiments. We propose an innovative and sensitive means to identify and characterize these changes in cell lines. Our technology will impact public health by making studies based on cell culture more efficient, reliable and easier to interpret.
Project Terms: Alpha Cell; base; Binding; Cell Culture Techniques; Cell Line; Cells; Custom; Data; Development; Devices; DNA; epigenomics; Evolution; experimental study; Funding; Future; Genome; genome analysis; Genomic DNA; Genomics; Haplotypes; Hybrids; In Vitro; Individual; Industrialization; innovation; instrument; interest; Lab-On-A-Chips; Length; Liquid substance; Molecular; Monitor; Oligonucleotides; Outcome; Output; Phase; phase 1 study; Plants; Population; prototype; Public Health; Research; Research Personnel; research study; single molecule; Stretching; Structure; success; Surface; System; Technology
