Emphysema is a prevalent chronic obstructive pulmonary disease (COPD) induced predominantly by cigarette smoking is defined as the combination of chronic bronchitis (in the airways) and emphysema, which characterized by excessive cell apoptotic cell death of alveolar lung structural cells and chronic inflammation. Because alveolar structures are essential for gas exchange in the lungs, loss of this tissue component in emphysema affects life quality and health dramatically. Current COPD therapy targets mainly the airways, and no treatment is available for halting loss of parenchymal lung tissue. The founders of EmphyMAb have made the discovery that pro-apoptotic and monocyte stimulating cytokine endothelial monocyte activating protein (EMAPPI) is upregulated in the human bronchoalveolar lavage of current-smokers and of COPD patients who were ex-smokers. Enhanced levels of EMAPII in the murine lung can be experimentally induced by exposing mice to cigarette smoke (CS); furthermore, lung-specific transgenic overexpression of EMAPII causes emphysematous pathology in mouse lung. The strong correlation between disease pathology and EMAPII levels suggested modulating levels of this cytokine might be a useful therapeutic intervention in emphysema. To this end, the EmphMAb team developed an EMAPII specific neutralizing rat monoclonal antibody (mAB), which abrogated functional and morphometric manifestations of CS-induced emphysema in mice when administered either concomitantly or after prolonged CS exposure. The team succeeded in generation of a partially humanized chimeric antibody with only slightly less affinity and activity in comparison to the parental one. In this Phase I application, EmphyMAb and its partners at Indiana University plan to fully humanize and increase affinity of the MAb, and determine that the bioactivity and affinity of this recombinant antibody are better or at least equivalent to the original MAb. The studies proposed here, will be the basis for future goals of establishing a highly specific fully humanized EMAP II mAB for use in human COPD patients as a first treatment for halting the progressive loss of gas exchange permitting lung tissue.
Public Health Relevance Statement: Public Health Relevance: There is an urgent need for effective treatments to address the progressive tissue loss in emphysema as the most fatal component of chronic obstructive pulmonary disease (COPD). Current treatment mostly targets the airway and cannot halt lung tissue destruction, which when progressive finally leads to the need of oxygen treatment and/or prolonged hospitalization. EmphyMAb and its research partners at Indiana University have shown that the protein endothelial monocyte activating protein (EMAP II) is induced in human and mouse lungs upon cigarette smoke exposure and causes lung emphysema in mice. The team has developed a monoclonal antibody to EMAP II that blocks lung emphysema formation and progression in animal disease models. Based on these encouraging results, EmphyMAb are finalizing the humanization and increasing the potency of this antibody in preparation for studies to examine safety in animals, which will allow us to proceed to testing in human subjects and, ultimately, COPD patients.
NIH Spending Category: Biotechnology; Chronic Obstructive Pulmonary Disease; Emphysema; Lung; Prevention; Smoking and Health; Tobacco
Project Terms: Address; Adrenal Cortex Hormones; Affect; Affinity; Alveolar; Amino Acid Sequence; Animal Disease Models; Animals; Antibodies; Antibody Affinity; Apoptotic; base; Binding (Molecular Function); Biological Models; Bronchoalveolar Lavage; Bronchodilator Agents; Cell Death; Cells; Cessation of life; chimeric antibody; Chronic; Chronic Bronchitis; Chronic Obstructive Airway Disease; cigarette smoke; cigarette smoke-induced; Cigarette smoke-induced emphysema; cigarette smoking; clinical application; Controlled Clinical Trials; cytokine; Development; Disease; Disease Progression; effective therapy; Endothelial Cells; endothelial monocyte-activating polypeptide II; environmental tobacco smoke exposure; Epidemic; Epitopes; Funding; Future; Gases; Generations; Goals; Health; Hospitalization; Human; human subject; humanized antibody; Indiana; Inflammation; intravenous administration; Investigational Drugs; Lead; Lung; lung injury; Maps; Marketing; Mediator of activation protein; Medical; Modeling; Monoclonal Antibodies; monocyte; Mortality Vital Statistics; Mus; Mutate; neutralizing antibody; novel; novel therapeutics; overexpression; Oxygen; Parents; Pathology; Patients; Pharmaceutical Preparations; Phase; phase 2 study; pre-clinical; Preparation; Prevalence; Process; Property; Proteins; public health relevance; Pulmonary Emphysema; Quality of life; Rattus; Recombinant Antibody; Research; Rodent; Route; Safety; Site; Small Business Innovation Research Grant; Smoker; smoking cessation; Staging; Structure; Structure of parenchyma of lung; success; Supplementation; targeted treatment; Testing; Therapeutic; Therapeutic antibodies; Therapeutic Intervention; Tissues; tool; Toxic effect; Transgenic Organisms; United States Food and Drug Administration; Universities; Validation; Wood material
