Microfabricated chemical and biological sensors serve as an interface between biologic and electronic systems. Previously, through TATRC-funded research grants (W81XWH-05-2-0064, W81XWH-10-1-0358), we have investigated the behavior and detection of propofol using electrochemical (EC) methods. Using these analytical methods, we have quantified the oxidation of propofol well below therapeutic concentrations in reagents and in serum, and have designed and fabricated several prototype biosensors using; 1) carbon fiber microdisc electrodes, 2) carbon nanofiber electrodes, and 3) organic membrane-coated carbon electrodes. We have previously tested and reported on sensor performance, signal sensitivity and stability, biofouling, and biological interference ex vivo to model clinical performance and validated sensor performance in pilot clinical studies. We have also developed a prototype preclinical platform feedback control loop (including controller design elements) for closed-loop infusional propofol anesthesia to automate drug delivery in real-time in vivo, using small infusion systems. The goal of this Phase I SBIR is to integrate our biosensor prototypes and EC methods with current TCI pump technology and complete controller design testing of the small platform closed loop device to deliver real time (<1 second) sensing of propofol levels.
Keywords: Biosensors, Closed Loop Anesthesia, Propofol, Electrochemistry, Prototyping, Automated Anesthesia, Controller Theory, Nanotechnology
