The current SBIR Phase I proposal addresses a need for the rapid and simple sample preparation of genomic DNA from human saliva and blood through a novel technology: microscale tip enrichment probes that employ electric fields and capillary action. With the action of a microtip-installed device, DNA contained in a given saliva sample can be selectively captured without centrifugation on the proposed microtip, and stored as a dried form for long- term preservation and delivery. The preserved DNA on microtips is released into a desired medium for PCR analysis without additional purification steps. Therefore, this innovative technology enables the one-step concentration of DNA from human saliva and blood as a purified, durable form. This application describes a feasibility study of the proposed method and system to explore and optimize the protocol of centrifuge-free preparation of PCR-ready DNA.
Public Health Relevance: This proposal aims to conduct a feasibility study for a portable device capable of concentrating and purifying human genomic DNA from saliva without centrifuge. The success of the project would provide a convincing rationale and form a technical basis for the proposed methodology for rapid and simple preparation of PCR-ready DNA from various sample specimens with a high throughput at a low cost.
Public Health Relevance Statement: This proposal aims to conduct a feasibility study for a portable device capable of concentrating and purifying human genomic DNA from saliva without centrifuge. The success of the project would provide a convincing rationale and form a technical basis for the proposed methodology for rapid and simple preparation of PCR-ready DNA from various sample specimens with a high throughput at a low cost.
NIH Spending Category: Bioengineering; Genetics
Project Terms: Address; base; Biological Preservation; Blood; Blood capillaries; Blood specimen; Body Fluids; capillary; Centrifugation; Clinical; Collection; commercialization; cost; Data; Development; Devices; Disposable Equipment; DNA; DNA analysis; electric field; Environment; Feasibility Studies; Genetic; Genomics; Goals; Human; human DNA; Human Genetics; innovative technologies; Laboratories; Methodology; Methods; new technology; Normal Statistical Distribution; One-Step dentin bonding system; Performance; Phase; Plasma; Preparation; Protocols documentation; prototype; Public Sector; Reagent; Recovery; Reproducibility; Risk; Running; Saliva; Sampling; Site; Small Business Innovation Research Grant; Source; Specimen; Spottings; Staging; success; Swab; System; Testing; Time; United States National Institutes of Health; Whole Blood; Work
